Nitrogen (N) is a mineral nutrient that is essential for the normal growth and development of plants that is required in the highest quantity. It is an element of nucleic acids, proteins, and photosynthetic metabolites, therefore crucial for crop growth and metabolic processes. Recently, it was estimated that N fertilizers could meet the 48% demand of the world’s population. However, overuse and misuse of N fertilizers raised environmental concerns associated with N losses by nitrous oxide (N2O) emissions, ammonia (NH3) volatilization, and nitrate (NO3−) leaching. For instance, NH3 is a pollutant in the atmosphere, N2O is a greenhouse gas that has a warming potential 298 times higher than CO2 and contributes to ozone depletion, and NO3− causes eutrophication of water bodies. Agricultural practices account for about 90% of NH3 and 70% of N2O anthropogenic emissions worldwide. The efficient use of N chemical fertilizers can be attained through cultural and agronomic practices. Nitrogen use efficiency (NUE) is an important trait that has been studied for decades in different crops. The grain production or economic return from the per unit supply of N fertilizer simply explained the NUE. Several definitions were suggested by different researchers. NUE can be defined as the product of N uptake efficiency (NUpE) and N utilization efficiency (NUtE). An increase in NUE increases the yield, biomass, quality, and quantity of crops. N is generally applied as chemical fertilizer to the soil, whereas a small amount is added to some crops like grain legumes through the fixation process. On the other hand, crop plants take N through the root system in the form of nitrate or ammonium which is thereby used in different metabolic processes. A number of studies have been conducted to increase the NUE in different crops and it has been indicated that NUE can be improved by agronomic, physiological, biochemical, breeding as well as molecular approaches. Nitrogen is the main limiting nutrient after carbon, hydrogen, and oxygen for the photosynthetic process, phyto-hormonal and proteomic changes, and the growth-development of plants to complete their lifecycle. Excessive and inefficient use of N fertilizer results in enhanced crop production costs and atmospheric pollution. Atmospheric nitrogen (71%) in the molecular form is not available for the plants. For the world's sustainable food production and atmospheric benefits, there is an urgent need to upgrade nitrogen use efficiency in the agricultural farming system. Nitrogen losses are too high, due to excess amount, low plant population, poor application methods, etc., which can go up to 70% of total available nitrogen. These losses can be minimized up to 15–30% by adopting improved agronomic approaches such as optimal dosage of nitrogen, application of N by using canopy sensors, maintaining plant population, drip fertigation, and legume-based intercropping. Therefore, the major concern of modern days is to save economic resources without sacrificing farm yield as well as the safety of the global environment, i.e. greenhouse gas emissions, ammonium volatilization, and nitrate leaching.