Peptide Nucleic Acids

Peptide Nucleic Acids
Author: Peter E. Nielsen
Publisher: Humana Press
Total Pages: 274
Release: 2002-07-23
Genre: Science
ISBN: 9780896039766

Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA—an uncharged pseudopeptide—that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA—many of which have not yet been validated— I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .

Peptide Nucleic Acids

Peptide Nucleic Acids
Author: Peter E. Nielsen
Publisher: Garland Science
Total Pages: 332
Release: 2004
Genre: DNA
ISBN: 9780954523244

Peptide Nucleic Acids, Second Edition has been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.

Delivery Technologies for Biopharmaceuticals

Delivery Technologies for Biopharmaceuticals
Author: Lene Jorgensen
Publisher: John Wiley & Sons
Total Pages: 442
Release: 2009-10-23
Genre: Science
ISBN: 0470688408

Advances in biotechnology have provided scientists with an increasing number of biopharmaceuticals such as novel peptide and protein drugs as well as nucleic acid based drugs for gene therapy. However, successful delivery of these biopharmaceuticals is a major challenge because their molecular properties lead to poor physical and chemical stability in the body and limited membrane permeability. Therefore researchers are developing a range of new delivery technologies and materials to enable these new drugs to be delivered intact to their target sites. Delivery Technologies for Biopharmaceuticals describes strategies to overcome the main barriers for successful delivery of therapeutic peptides, proteins, and nucleic acid-based drugs or vaccines related to the site of administration and the target site. Many of the approaches described are reported in formulations in current clinical trials as well as in marketed products. Contents include: challenges in delivery of biopharmaceuticals novel formulation approaches for peptide and protein injectables non-viral chemical vectors and viral technology for delivery of nucleic acid based drugs immune response, adjuvants and delivery systems for vaccines several examples of delivery systems for different biopharmaceuticals a critical assessment of delivery technologies for biopharmaceuticals Delivery Technologies for Biopharmaceuticals is an essential single-volume introduction to the technologies used by researchers to ensure efficient delivery of this exciting new class of drugs. It will be of value to researchers and students working in drug delivery, formulation, biopharmaceuticals, medicinal chemistry, and new materials development.

Diagnostic Molecular Biology

Diagnostic Molecular Biology
Author: Chang-Hui Shen
Publisher: Elsevier
Total Pages: 590
Release: 2023-06-29
Genre: Science
ISBN: 0323986099

Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. - Provides explanations on how techniques are used to diagnosis at the molecular level - Explains how to use information technology to communicate and assess results in the lab - Enhances our understanding of fundamental molecular biology and places techniques in context - Places protocols into context with practical applications - Includes extra chapters on respiratory viruses (Corona)

Nucleic Acids Chemistry

Nucleic Acids Chemistry
Author: Ramon Eritja
Publisher: Walter de Gruyter GmbH & Co KG
Total Pages: 380
Release: 2021-01-18
Genre: Science
ISBN: 311063953X

This book compiles recent research on the modification of nucleic acids. It covers backbone modifications and conjugation of lipids, peptides and proteins to oligonucleotides and their therapeutic use. Synthesis and application in biomedicine and nanotechnology of aptamers, fluorescent and xeno nucleic acids, DNA repair and artificial DNA are discussed as well.

Nanomaterials for Biosensors

Nanomaterials for Biosensors
Author: Bansi D. Malhotra
Publisher: William Andrew
Total Pages: 334
Release: 2017-10-26
Genre: Technology & Engineering
ISBN: 0128135158

Nanomaterials for Biosensors: Fundamentals and Applications provides a detailed summary of the main nanomaterials used in biosensing and their application. It covers recent developments in nanomaterials for the fabrication of biosensor devices for healthcare diagnostics, food freshness and bioprocessing. The various processes used for synthesis and characterization of nanostructured materials are examined, along with the design and fabrication of bioelectronic devices using nanostructured materials as building blocks. Users will find the fundamentals of the main nanomaterials used in biosensing, helping them visualize a systematic and coherent picture of how nanomaterials are used in biosensors. The book also addresses the role of bio-conjugation of nanomaterials in the construction of nano-biointerfaces for application in biosensors. Such applications, including metal nanoparticles, metal oxide nanoparticles, nanocomposites, carbon nanotubes, conducting polymers and plasmonic nanostructures in biosensing are discussed relative to each nanomaterial concerned. Finally, recent advancements in protein functionalized nanomaterials for cancer diagnostics and bio-imaging are also included. - Provides a detailed study on how nanomaterials are used to enhance sensing capabilities in biosensors - Explains the properties, characterization methods and preparation techniques of the nanomaterials used in biosensing - Arranged in a material-by-material way, making it clear how each nanomaterial should be used

Current Protocols in Nucleic Acid Chemistry

Current Protocols in Nucleic Acid Chemistry
Author: Serge L. Beaucage
Publisher: Current Protocols
Total Pages:
Release: 2000
Genre: Science
ISBN: 9780471246626

Good methods must be reliable, well-tested, and honed to minimize the time and expense required to achieve the desired results. CPNC provides a continuously growing and evolving set of protocols that allows researchers to benefit from the experience of other researchers around the world. The core manual provides a comprehensive set of protocols that have been compiled, revised, and streamlined over the last 6 years. Quarterly updates provide new protocols in emerging areas of research as well as continued advances and new applications for fundamental methods. The book is designed to grow and change with the field of nucleic acid chemistry. Fundamental nucleoside chemistry methods include sugar-base condensation, phosphorylation, and nucleoside protection. Methods for oligonucleotide synthesis include H-phosphonate and phosphoramidite approaches, solid-phase and solution-phase synthesis, large-scale synthesis, synthesis for modified and unmodified oligonucleotides, conjugation of oligonucleotides, synthesis without base protection, and synthesis on microarrays. More specialized synthetic methods include synthesis of biologically active nucleosides and prodrugs. Purification and characterization methods are detailed. Advanced methods include biophysical analysis, combinatorial methods, and nanotechnology. Each protocol includes rationale for choosing appropriate methods, step-by-step procedures, complete recipes, anticipated results, characterization data, and troubleshooting, as well as background and recommended reading. The level of procedural detail is far beyond that found in the research literature, and tips and comments from authors are geared towards ensuring reliable duplication in the laboratory.

Antisense Research and Application

Antisense Research and Application
Author: Stanley T. Crooke
Publisher: Springer Science & Business Media
Total Pages: 646
Release: 2012-12-06
Genre: Medical
ISBN: 3642587852

Antisense technology may result in dramatic changes in the therapy of many diseases and may provide tools to dissect pharmacological processes and to confirm the roles of various genes. In this volume, progress in the understanding of antisense technology and its use in creating new drugs is discussed. Potential caveats, pitfalls and limitations of the technology are also presented. In the next few years the pace at which new molecular targets will be identified will increase exponentially as the sequencing of the human genome and of other genomes proceeds.

Nucleic Acids

Nucleic Acids
Author: Victor A. Bloomfield
Publisher: Sterling Publishing Company
Total Pages: 854
Release: 2000-04-17
Genre: Science
ISBN: 9780935702491

Providing a comprehensive account of the structures and physical chemistry properties of nucleic acids, with special emphasis on biological function, this text has been organized to meet the needs of those who have only a basic understanding of physical chemistry and molecular biology.