Author | : Edwin F. George |
Publisher | : |
Total Pages | : |
Release | : 1993 |
Genre | : Plant micropropagation |
ISBN | : 9780950932545 |
Author | : Edwin F. George |
Publisher | : |
Total Pages | : |
Release | : 1993 |
Genre | : Plant micropropagation |
ISBN | : 9780950932545 |
Author | : Robert H. Smith |
Publisher | : Academic Press |
Total Pages | : 190 |
Release | : 2012-12-02 |
Genre | : Science |
ISBN | : 0323160476 |
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Author | : A.K. Hvoslef-Eide |
Publisher | : Springer Science & Business Media |
Total Pages | : 578 |
Release | : 2005-06-15 |
Genre | : Science |
ISBN | : 1402032005 |
High-efficiency micropropagation, with relatively low labour costs, has been demonstrated in this unique book detailing liquid media systems for plant tissue culture. World authorities (e.g. von Arnold, Curtis, Takayama, Ziv) contribute seminal papers together with papers from researchers across Europe that are members of the EU COST Action 843 "Advanced micropropagation systems". First-hand practical applications are detailed for crops – including ornamentals and trees – using a wide range of techniques, from thin-film temporary immersion systems to more traditional aerated bioreactors with many types of explant – shoots to somatic embryos. The accounts are realistic, balanced and provide a contemporary account of this important aspect of mass propagation. This book is essential reading for all those in commercial micropropagation labs, as well as researchers worldwide who are keen to improve propagation techniques and lower economic costs of production. Undergraduate and postgraduate students in the applied plant sciences and horticulture will find the book an enlightened treatise.
Author | : M. K. Razdan |
Publisher | : Science Publishers |
Total Pages | : 394 |
Release | : 2003 |
Genre | : Science |
ISBN | : 9781578082377 |
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.
Author | : D. F. Wetherell |
Publisher | : Avery Publishing Group |
Total Pages | : 106 |
Release | : 1982 |
Genre | : Health & Fitness |
ISBN | : |
Author | : John H. Dodds |
Publisher | : International Potato Center |
Total Pages | : 260 |
Release | : 1985-10-31 |
Genre | : Science |
ISBN | : 9780521315166 |
The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
Author | : Duong Tan Nhut |
Publisher | : Springer Nature |
Total Pages | : 400 |
Release | : 2022-04-05 |
Genre | : Science |
ISBN | : 9811664986 |
This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.
Author | : Mohammad Anis |
Publisher | : Springer |
Total Pages | : 616 |
Release | : 2016-10-08 |
Genre | : Science |
ISBN | : 9811019177 |
This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed.
Author | : Edward E Johnson |
Publisher | : |
Total Pages | : 130 |
Release | : 2020-12-23 |
Genre | : |
ISBN | : |
Do you want to know how to tissue culture plants and grow more in less space? If so this how-to guide is for you. Plant tissue culture can be done at home without expensive lab grade gear. Inside, you will find easy and affordable alternatives to supplies and equipment that would otherwise be unobtainable to most. The return in numbers of plants for your investment is very lucrative and rewarding, not to mention easy.Anyone that can cook dinner can practice micropropagation of plants in a compact space and in incredible numbers. Anyone that has seen the exploding price of houseplants and recreational plants can see what a reward growing thousands of plants yourself can bring.What you need to start a successful lab at home in a compact spaceHow to use your equipment and supplies as easily as possibleWhat each stage does and how to easily perform the tasksHow to get your favorite plants into tissue cultureWhy you should be using plant tissue culture to grow to your potentialHow to grow out your tissue cultured plants for outside or saleAquarium plants, houseplants, garden plants, recreational plants, carnivorous plants, orchids, mosses, and more can quickly and easily be multiplied. Many plants you see at garden centers are propagated by plant tissue culture and you can do it too!Turn one plant into thousands quickly. In the amount of time it takes to grow a cutting to produce new shoots to make more cuttings you can have hundreds of plants in many species. Plant tissue culture allows the multiplication of your prized plants exponentially. It also allows you to use a kitchen corner or a small room as a lab area that will give you positive results.Keep up with the demand and changing tastes of the plant hobby. Propagate plants faster with tissue culture and keep up with your demand for more plants.