Dung beetles (Coleoptera: Scarabaeidae) provide fundamental ecosystem functions and services, like nutrient cycling, bioturbation, secondary seed dispersal, parasite and fly control, and soil fertilization, but land use transformation, has negatively impacted their diversity and processes. For the last four decades, dung beetles have been used as one of the most crucial insect groups for analyzing and monitoring biodiversity in natural temperate and tropical ecosystems, and their anthropogenic ecosystem´s derivatives. Dung beetles seem to be declining mainly for the forest conversion to agrosystems and others ecosystems transformed by human activity in the Neotropical region. Our knowledge of the dung beetle responses to the transformation of their original habitat has increased over the last two decades in the Neotropical region. However, the knowledge on the taxonomy, ecology, biology, and the factors producing the anthropogenic activity on Neotropical dung beetles has not been met and analyzed in full. This Research Topic synthesizes the knowledge on the diversity, taxonomy, and biology of the dung beetle species in the Neotropical region. The structure of this Research Topic is composed of two sections. In the first section, articles may be original research papers or reviews on the knowledge of the dung beetles diversity in each country of the Neotropical region, including species diversity and their response to land use and habitat fragmentation. Articles on the second section may be original research papers or reviews on the following Research Topics: • Taxonomy of Neotropical dung beetles and their preservation in Institutional collections • The methodology used to analyze the spatial distribution and monitoring of dung beetles • The response of dung beetles to habitat loss and modification to the landscape in different countries and Neotropical biomes: Cloud forest, Tropical rain forest, Subtropical forest, Cerrado, Caatinga, Paramo, Pampa, Pantanal, and others • The physiological responses of dung beetles to anthropogenic disturbance in the Neotropics • The biology and reproductive behavior of Neotropical dung beetles • The genetics of Neotropical dung beetle • Dung beetle interaction with other species and its role as a secondary dispersal • The relationship between dung beetles and Mesoamerican cultures